What is tissue culture in agriculture



What are the applications of tissue culture in agriculture?

By late 70’s it was evident that plant tissue culture technique can be successfully used in various field of agriculture, such as, production of pathogen free culture, pro­duction of secondary products, clonal propagation, mutant culture, haploid breeding and genetic engineering. By tissue culture, pathogen free cultures have been produced.

What is tissue culture in microbiology?

Tissue culture is a technique in which fragments of plant or animal tissues are cultured and grown in a laboratory. Many times the organs are also used for tissue culture. The media used for the growth of the culture is broth and agar. This technique is also known as micropropagation.

What is the difference between plant and animal tissue culture?

Both plant and animal tissues can be used for culturing. For eg., animal tissue culture helps in preserving an organ or tissue. Plant tissue culture may be used for genetic modification of a plant or simply increase its yield. the cells of the plants can be genetically altered to produce plants with desirable characteristics.


What is a cultured tissue?

The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an organ. Cells in culture may multiply; change size, form, or function; exhibit specialized activity (muscle cells, for example, may contract); or interact with other cells.

When was tissue culture first used?

An early attempt at tissue culture was made in 1885 by German zoologist Wilhelm Roux, who cultivated tissue from a chick embryo in a warm salt solution. The first real success came in 1907, however, when American zoologist Ross G. Harrison demonstrated the growth of frog nerve cell processes in a medium of clotted lymph. French surgeon Alexis Carrel and his assistant Montrose Burrows subsequently improved upon Harrison’s technique, reporting their initial advances in a series of papers published in 1910–11. Carrel and Burrows coined the term tissue culture and defined the concept. Thereafter, a number of experimenters succeeded in cultivating animal cells, using as culture media a variety of biological fluids, such as lymph, blood serum, plasma, and tissue extracts. In the 1980s and ’90s, methods were developed that enabled researchers to successfully grow mammalian embryonic stem cells under artificial conditions. Those breakthroughs ultimately enabled the establishment and maintenance of human embryonic stem cell lines, which advanced researchers’ understanding of human biology and greatly facilitated progress in therapeutics and regenerative medicine.

How are cultures grown?

Cultures are usually grown either as single layers of cells on a glass or plastic surface or as a suspension in a liquid or semisolid medium. To initiate a culture, a tiny sample of the tissue is dispersed on or in the medium, and the flask, tube, or plate containing the culture is then incubated, usually at a temperature close to that …

What is the culture environment?

Culture environments. Cells may be grown in a culture medium of biological origin such as blood serum or tissue extract, in a chemically defined synthetic medium, or in a mixture of the two. A medium must contain proper proportions of the necessary nutrients for the cells to be studied and must be appropriately acid or alkaline.

What are the two types of cultures?

There are two main types of cultures: primary (mortal) cultures and cultures of established (immortal) cell lines. Primary cultures consist of normal cells, tissues, or organs that are excised directly from tissue collected by biopsy from a living organism. Primary cultures are advantageous in that they essentially model the natural function …

Why are primary cultures beneficial?

Primary cultures are advantageous in that they essentially model the natural function of the cell, tissue, or organ under study. However, the longer the samples are maintained in culture, the more mutations they accumulate, which can lead to changes in chromosome structure and cell function.

What is tissue culture hood?

Tissue culture often requires sterile working conditions and thus is typically performed in a laminar flow cabinet ( or tissue culture hood), which circulates filtered air to reduce the risk of culture contamination . © Punctum/Press and Information Office of the Federal Government of Germany. Britannica Quiz. All About Biology Quiz.

What is tissue culture?

Plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media under sterile and controlled conditions of light, temperature, and humidity.

What is tissue culture technique?

Plant tissue culture technique has become an important tool in the hands of the plant biotechnologists. A number of research investigations have been reported for the production of biologically active constituents using plant tissue culture techniques. Cassia senna Linn. (Caesalpineaceae) is an important medicinal plant, which has been widely used in Ayurveda. The active chemical components of the plant are anthraquinone glycosides – sennosides, especially sennosides A and B, which are responsible for the purgative action. A protocol for tissue culture of C. senna is established in different morphogenetic media and in vitro -grown tissues/cells were analyzed for their biosynthetic potential. 24 The results of the study indicate that the in vitro- cultured partially organized cells of C. senna inherited the biosynthetic potential, which can be exploited for production of sennosides on a large scale under proper growth conditions. The whole venture to explore the cultures of ayurvedic medicinal plants for bioactive constituents was undertaken all over the world and soon it blossomed into a new technology that has affected the phytochemical industry to a large extent. Commercial viability and economic feasibility still remain the decisive factors in the industrial production of such metabolites from the cultures. The range of metabolites produced by the callus and cell suspension cultures includes alkaloids, glycosides, flavonoids, and others. The cell suspension cultures are particularly capable of synthesizing such molecules and are regarded as potentially suitable systems for producing the metabolites of high economic value. They produce the bioactive molecules equivalent to or higher in yields to the plants from which they are derived. 25 Plant cell culture provides an alternative method for production of plant secondary metabolites.

What is auxin habituation?

He called this phenomenon auxin habituation. It was soon recognized that similar variation can occur for cytokinins and more rarely for some other hormones. Thus, habituation is when a culture continues to develop in the absence of auxin or cytokinin. For example, shoot cultures habituated for cytokinin would continue to produce new shoots on a cytokinin-free medium. Hormone habituation is a phenomenon by which plant cells and tissues lose the requirement of exogenous hormones to sustain cell division and development upon continuous culture [12].

What is the role of sucrose in plant tissue culture?

During plant tissue culture growth sucrose acts as a fuel source for sustaining photomixotrophic metabolism (organisms can use different sources of energy and carbon), ensuring optimal development , although other important roles such as carbon precursor or signaling metabolite have more recently been highlighted.

What is the concentration of sucrose in anther culture?

Hence, in anther culture a higher concentration of sucrose (6–12%) is used.

What are the facilities required for tissue culture?

These include areas for general washing and media preparation, sterilization, storage, aseptic transfer, observation/data collection, and environmentally controlled incubators or culture rooms.

Does autoclaving inhibit growth of carrots?

It has been found that a plant tissue culture medium containing glucose or fructose sterilized by autoclaving inhibits the growth of carrot root tissue cultures. More growth inhibition occurs when sugar and culture medium is autoclaved together [237].

What is tissue culture?

Tissue culture is used to develop thousands of genetically identical plants from one single parent plant known as somaclones, and this process is known as micropropagation. The method offers an advantage over other methods as it can be used to develop disease free plants from disease-rode plants by using their meristems (apical and axillary) as explants.

Why is tissue culture important?

Since the conventional breeding techniques could not fulfil the required demand of crops, tissue culture came around as a grand leap in breeding practices. It makes use of parts of a plant to generate multiple copies of the plant in a very short duration.

What is the part of a plant that is used for culturing?

The part (s) of the plant used for culturing is known as explants. The explants are cultured in-vitro on a nutrient medium that caters to fulfil its nutritional requirements. The nutrient medium must provide the following:-

What is culture media?

Apart from these, the culture media also serves as a medium for supplying phytohormones or plant growth regulators to the issues which bring about their morphogenesis as per requirement. The tissues of the explants first lose their specificity to form a hard brown lump known as callus.

What is tissue culture?

Tissue culture is a technique of growing new plants tissue by transferring them into an artificial environment in which they can continue and function. This article deals with tissue culture, process how it is done, its importance and advantages.

Why is tissue culture important?

So, we have understood that tissue culture is a technique which is important for transforming plants with new genes.

What are the advantages of tissue culture?

Advantages of Tissue Culture. 1. Tissue culture is a very fast technique. Thousands of plantlets can be produced in a few weeks time from a small amount of plant tissue. 2. The new plants produced by tissue culture are disease free. 3. Tissue culture can grow plants round the year, irrespective of weather or season. 4.

How does tissue culture produce new plants?

The process of tissue culture for producing new plants is as follows: 1. A small piece of plant tissue is taken from the growing point of the plant or from the tip of the plant and placed on a sterile jelly which contains nutrients and plant hormones. The hormones make the cells in the plant tissue divide rapidly producing many cells which forms …

Why is growth medium important?

In this process the growth medium or culture solution is very important as, it is used for growing plant tissue because it contains various plant nutrients in the form of ‘jelly’ known as agar and plant hormones which are necessary for the growth of plant. 1.

How are seeds produced?

We know that seeds are produced due to sexual reproduction of plants and each seed has its own genetic material which is unique from other seeds and also from the parent plant. Generally, tissue culture plants are micro propagated cuttings or clones, genetically identical to the mother and all the daughter plants.

How are plantlets produced?

5. The plantlets thus produced are transplanted into pots or soil where they can grow to form mature plants.

What is tissue culture?

Tissue culture has been playing a very significant role in regeneration, mass multiplication, and propagation of whole plant from transformed or genetically modified cells or tissues. This is one of the most important steps in tissue culture-based genetic engineering program.

What is the role of tissue culture in plant breeding?

Tissue culture has played a very significant role in the field of plant breeding. Plant breeding creates genetic variability and tries to tap the beneficial variations in a population or living organisms.

What is the role of PTC in agriculture?

PTC also plays a pivotal role in industry, agriculture, and plant breeding as it complements crop production through micropropagation, synthetic seed formation, somaclonal variation, hybridization, genetic transformation, haploid culture, pathogen eradication, and by preserving germplasm.

What is hairy root culture?

Hairy root culture is the culture raised after the infection of explants/cultures by the gram negative soil bacterium A . rhizogenes ( Tepfer and Casse-Delbart, 1987 ). It is also known as transformed root culture, which is used to study plant metabolic processes, producing valuable secondary metabolites or recombinant proteins; it also helps in the analysis of gene ( Georgiev et al., 2007 ). In this process, wounded explants are first inoculated with A. rhizogenes and after two or three days the explants are transferred to solid media with antibiotics, for example, cefotaxime for short period of time. Hairy root culture depicts a remarkable characteristic for a successful high scale-up biomass growth, stable production of secondary metabolites, sensitive to external stimuli and metabolite release in culture medium.

What are the different types of organ culture?

Most important types of organ culture for in vitro plant propagation are meristematic culture, shoot tip, nodal culture of separate lateral bud, isolated root, and embryo culture. In vitro organ culture and metabolic composition of chemicals have been established in several plants like Fritillaria unibracteata.

How does genetic variability affect plant breeding?

Genetic variability has a huge role in the success of any plant breeding program. With the advent of new technologies such as PTC and recombinant DNA technology, a lot of progress has been made in increasing food production. Somaclonal variations are genetic alterations developed under in vitro stressed microenvironment. These could be epigenetic or nonchanges that may be heritable or nonheritable. Somaclonal variations lead to various genetic changes in plants, which can be used to change various plant characters such as plant height, yield, and grain quality and provide resistance to plants against diseases, pests, and drought ( Patnaik et al., 1999 ). With change in all these characters, plant production can increase to counter the demand of alarming increase in population. Somaclonal variations have been reported in various varieties of different crop plants such as apple ( Rosati and Predieri, 1990 ), potato ( Das et al., 2000 ), banana ( James et al., 2007 ), Heliconia bihai ( Rodrigues, 2008 ), and sugarcane ( Sengar et al., 2009 ). Somaclonal variations in sugarcane were helpful in increasing its sugar yield and improving the resistance to eye-spot disease ( Sengar et al., 2009 ). Similarly, in potato, it was used to improve its tuber shape and uniformity, and provide resistance against late blight disease ( Das et al., 2000 ). Various new traits were recovered, which were not present in natural gene pool such as glyphosate resistance in tobacco, atrazine resistance in maize, increasing methionine and lysine contents in cereals and fungal resistance in alfa alfa ( Sengar et al., 2009 ).

What is tissue culture?

Tissue culture is the method of ‘in vitro’ culture of plant or animal cells, tissue or organ – on nutrient medium under aseptic conditions usually in a glass container. Tissue culture is sometimes referred to as ‘sterile culture’ or ‘in vitro’ culture.

Why is tissue culture important?

Tissue culture has great importance in studies of plant morphogenesis, physio­logy , biochemistry, pathology, embryology, cytology etc. From tissue culture studies it is possible to know bow simple cells differentiate and become specialized to perform special functions. Various changes taking place in a cell can be noted from clonal culture.

What is the method of culture of Nicotiana and Datura?

It was noted that single cells are capable of producing new plants. From pollen and anther culture haploid embryos were obtained. A method of microspore culture of Nicotiana and Datura was developed by Nitsch (’74, ’77). He was able to double the chromosome number and obtained homozygous diploid plants.

What is the term for a single cell culture?

Culture of single cell is sometimes called single cell cloning. The portion of the plant to start the culture is called an explant. Culture derived from a single explant is called a clone. In order to maintain a culture for a comparatively longer period the culture me­dium is changed from time to time.

What are the substances that are used in vitro culture?

Some of these substances are enzymes, vitamins, food flavours, sweeteners, anti-tu­mour alkaloids and insecticides. In Japan ‘in vitro’ culture has been achieved at industrial level. Clonal propagation of orchids and several other ornamental and economic plants have been achieved by ‘in vitro’ culture.

When was meristematic tissue cultured?

Culture of meristematic tissue was started in early 20th century. Isolated root tips were first cultured by Robbin in 1922. Working independently Kotte (’22) also made similar observations. Robbin and Maneval (’23) cultured roots and maintained the culture for 20 weeks by sub culturing.

Who said that cells could be cultured outside the body of the organism?

In 1832 Theodor Schwann said that cells could be cultured outside the body of the organism if provided with proper external conditions. In 1835 Wilhelm Roux cultured embryonic cells of chicken in salt solution. Reichinger (1839) said that fragments thicker than 1.5 mm were capable of growth but fragments below this limit failed to grow. He did not used any nutrient in his experiment.

What is tissue culture made of?

The media for tissue culture plants is made up of macronutrients, micronutrients, vitamins, growth regulators, and carbohydrates or sucrose. These elements are common for most of the plants and are designed by Murashige and Skoog. Some special groups of plants like conifers may require additional nutrients.

Why is tissue culture important?

The market for an improved variety of plants can be slow if the multiplication rate is slow. Tissue culture can help speed up this process by means of faster propagation. The use of chemicals is also reduced because the plants are grown in a disease-resistant environment.

What is the stage of plant growth?

This is the stage in tissue culture where the shoots or the plantlets that were grown are stimulated for root growth and hardening. It is also done within the internal environment such as a test tube. After the micropropagation process, root growth is an essential requirement and doesn’t happen in the early stages. The plantlets are put in a medium which stimulates growth and root initiation such as Auxin.

What are the advantages of tissue culture?

Advantages Of Tissue Culture Plants: A large number of plantlets can be grown in less time and space with a single explant. Tissues of plants are influenced by other plant correlative controls which can be isolated in this technique. The same replica of plants is created to produce good fruit and flower-bearing plants.

What stage of the plant is the tissue proliferating?

Here the tissue proliferates and produces new shoots. Stage 3 or the root formation: Hormones are used to induce rooting and the explants are prepared to be transferred to the regular growing media. Stage 4 or the establishment phase: The propagules are finally moved to the external soil conditions.

What is the part of a plant that is used for tissue culture called?

The part of the plant which is used for tissue culture is called an ‘explant ’. This culture was first established by Gottlieb Haberlandt in the year 1902.

What is the capacity of a plant cell to regenerate into a whole plant?

The capacity of a plant cell to regenerate into a whole plant is biologically termed as ‘cellular totipotency ’. The explants are grown in containers with a medium that is rich in nutrients. All the materials related to the tissue culture are sterilized before use to prevent infections and rotting of the tissue.

What is tissue culture?

In the current day and age, plant tissue culture is a popular scientific technique being followed by curious kitchen gardeners to renowned scientists from laboratories around the globe. It is both an art and science, in which multiplication of plant cells, tissues, organs, protoplasts and so forth, is done to form whole plants.

What are the characteristics of tissue culture?

One of the fascinating features of tissue culture is that each plantlet continues to produce and maintain small stems and leaves throughout its duration. Also, each plantlet developed is identical to other plantlets and to the mother plant, unlike the non- identical plants produced from seeds or cuttings.

How do plants grow?

There are several methods developed for growing plants. For instance, we already know that plants grow in fields from seeds. Then there are other methods like vegetative propagation where stem cuttings, tubers, etc. of different plants are used to grow and propagate in soil. These methods in general take a longer duration to grow …

Why is it important to have a sterile environment for plants?

For the successful development of healthy plantlets, it is necessary to create a sterile environment, free from living contaminants such as microorganisms, mites, insects and so on. As conventional plants growing in field conditions, these ‘greenhouses’ are quite susceptible to get fungal or bacterial infections and die due to diseases. In order to prevent this scenario , a balanced diet of chemicals is also supplied with biological antibiotics when required.

Does tissue culture take longer to grow?

These methods in general take a longer duration to grow with specific soil conditions that need to be present along with other factors. This is where tissue culture steps in to enhance the growth without the above-mentioned restrictions. It may sound complicated, however, tissue culture is an easy-to-follow process.

Is tissue culture used in cannabis?

In a nutshell, tissue culture has been widely used in several industries for decades now. However, for many industries like one of those booming now, for example, the cannabis industry, plant tissue culture is being adapted for large scale productions of good quality end products. Hence, there is extensive research going on in order …


Types of Plant Tissue Culture

  1. Seed Culture
  2. Embryo Culture
  3. Callus Culture
  4. Organ Culture

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Procedure of Plant Tissue Culture

  • The part(s) of the plant used for culturing is known as explants. The explants are cultured in-vitro on a nutrient medium that caters to fulfil its nutritional requirements. The nutrient medium must provide the following:- 1. Macronutrients – This includes elements like nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S) which is required for proper growth and morphogene…

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Uses of Plant Tissue Culture

  • Tissue culture is used to develop thousands of genetically identical plants from one single parent plant known as somaclones, and this process is known as micropropagation. The method offers an advantage over other methods as it can be used to develop disease free plants from disease-rode plants by using their meristems (apical and axillary) as exp…

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